Figure 6.

Smaller and fewer pancreatic islets in association with hyperglycemia in animals with islet-specific TonEBP deficiency. (A) Representative hematoxylin and eosin (H&E) stained images of pancreatic islets in TonEBP^fl/fl; NGN3 cre^+/− mice and their TonEBP^fl/fl littermates. (B) Number of pancreatic islets per mm² of tissues sections are shown in (A). Pancreas weight (C), body weight (D), plasma glucose (E) and plasma insulin (F) were analyzed from 14 week old animals: TonEBP^fl/fl; NGN3 cre^+/− mice (blue, n = 5) and their TonEBP^fl/f littermates (red, n = 8). (G) Representative fluorescence microscopic images showing BiP from TonEBP^fl/fl; NGN3 cre^+/− mice and their TonEBP^fl/f littermates. n represents the number of biologically independent animals. Mean + SE, * p < 0.05 ((B–F); unpaired t-test).