Table 3.

Mass spectrometry analysis confirms that DJ-1, interacting with histones, carries a peculiar phosphorylation on Threonine included in the Akt consensus. In the table are: Accession number, Univocal code identifying the protein on the Uniprot database (https://www.uniprot.org); Description of identified protein; Coverage, percentage of amino acid identified by MS/MS analysis; Unique peptides, number of peptides uniquely identifying the protein; PSMs, total number of identified peptide spectra matched for the protein; Score of identification; MW, molecular weight of identified protein; Calc pI, theoretical isoelectric point of identified protein; Sequence of identified peptide; PSMs, total number of identified peptide spectra matched for the peptide; Modification, position of modified residue and type of modification; MH+ Molecular weight of positive ion; Miss cleavage, number of allowed missed cleavages. White row is referred to protein MS data. Blue rows are referred to peptide MS data.

Accession	Description	Coverage	Unique Peptides	PSMs	Score	MW (kDa)	pI
Q99497	Protein DJ-1	64.55	11	58	102.40	19.9	6.79
	Sequence	PSMs	Modifications		MH+ (Da)	Mis Cleav.
Peptides	EGPYDVVVLPGGNLGAQNLSESAAVK	2			2584.3	0
	VTVAGLAGKDPVQCSR	6	C14(Carbamidomethyl)		1657.8	1
	VTVAGLAGKDPVQCSR	2	C14(Carbamidomethyl); R16(Methyl)		1671.8	1
	DKMMNGGHYTYSENR	3	M3(Oxidation); M4(Oxidation)		1834.7	1
	DVVICPDASLEDAKK	6	C5(Carbamidomethyl)		1659.8	1
	GAEEMETVIPVDVMR	6	M5(Oxidation); M14(Oxidation)		1707.7	0
	VTVAGLAGKDPVQCSR	1	C14(Carbamidomethyl); R16(Deamidated)		1658.8	1
	VEKDGLILTSR	2			1230.7	1
	MMNGGHYTYSENR	2	M1(Oxidation); M2(Oxidation)		1591.6	0
	VTTHPLAK	8			866.5	0
	VTVAGLAGK	4			815.5	0
	DGLILTSR	5			874.5	0
	EILKEQENR	6			1158.6	1
	ALVILAK	1			727.5	0
	VTTHPLAK	2	T3(Phospho)		946.4	0