Table 3.
Growth and exopolymer yields of Paenibacillus amylolyticus strain 2B and Pseudomonas extremaustralis strain 2ASCA in the presence of single carbon sources.
| Microorganism | Carbon Sources | O.D.* at λ540 nm | Fraction Yield: mg EP/L |
Carbohydrate Content (%. w/w) |
Protein Content (%. w/w) |
|---|---|---|---|---|---|
| Paenibacillus amylolyticus strain 2B | galactose | 1.490 | S: 68 | 0.0 | 1.4 |
| LB: 30.4 | 1.5 | 2.83 | |||
| TB: 113.2 | 21.2 | 5.98 | |||
| sucrose | 1.232 | S: 184 | 12 | 0.69 | |
| LB: 40.4 | 8.9 | 5.21 | |||
| TB: 123.2 | 11.1 | 18.63 | |||
| Pseudomonas extremaustralis strain 2ASCA | galactose | 1.054 | S: 167.6 | 2 | 0.23 |
| LB: 28.8 | 2.6 | 0.75 | |||
| TB: 47.2 | 26.6 | 3.3 | |||
| glucose | 1.246 | S: 275.2 | 1.1 | 0.52 | |
| LB: 51.2 | 6 | 0.63 | |||
| TB: 77.6 | 12.13 | 3.23 | |||
| mannose | 1.061 | S: 135.2 | 3.3 | 0.6 | |
| LB: 27.6 | 2.8 | 0.7 | |||
| TB: 21.2 | 25 | 3.4 | |||
| sucrose | 1.430 | S: 158.4 | 3.99 | 0.63 | |
| LB: 613.2 | 86.02 | 0.15 | |||
| TB: 34.8 | 18.65 | 3.53 |
All values are averages of two determinations. Uronic acid content in all exoproducts ranged from 0.02 to 2.0% w/w. * Optical density measured after four days of incubation at 10 °C under agitation (130 rpm). S: polymer in extracellular fraction. LB: polymer in loosely bound fraction. TB: polymer in tightly bound fraction.