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. 2020 Aug 28;10(9):1251. doi: 10.3390/biom10091251

Figure 6.

Figure 6

E100 decreased iba-1-positive microglial cells in cerebellum tissues of BTBR mice. (A) Immunofluorescent staining of cerebellar brain tissues with Iba-1 of BTBR mice; (A–D) Images show 20-μM-thick sections of cerebellum of different groups; (E) Densitometric assessment of fluorescent intensity was carried out to quantify activated microglia by Image J. (B,E) Significant increase (* p < 0.05) in the number of activated microglia was observed in cerebellum of BTBR mice compared to Ctrl mice. (C,E) E100 (5 mg/kg, i.p.) subchronic treatment significantly reduced (# p < 0.05) activated microglia in BTBR mice. (D,E) Co-injection of RAM (10 mg/kg, i.p.) for 21 days significantly reversed E100 (5 mg)-provided mitigation of activated microglia in BTBR mice as evidenced by the increased Iba-1-positive cells expression. Data expressed as the percent mean ± SEM (n = 3).