Figure S4.

NVJs impact the viability of nup133Δ cells, and Erg6 impacts NPC assembly mutant viability. (A) BODIPY-stained strains grown at 30°C. Scale bar, 5 µm. (B) Serial dilutions of GFP-nic96 mdm1Δ nvj1Δ dga1Δ lro1Δ or nup116ΔGLFG GFP-nic96 mdm1Δ nvj1Δ dga1Δ lro1Δ cells transformed with pRS315-NUP116 or pRS315, grown in synthetic complete leu and then plated onto YPD plates. (C–E) Serial dilutions of cells on YPD plates. LDO45 is required for loading of Pdr16 into LDs (Eisenberg-Bord et al., 2018; Teixeira et al., 2018). (F) Percentage of cells with GFP-Nic96 focus grown at 30°C. Error bars represent SD. *, P ≤ 0.01 compared with wild type using an unpaired two-tailed Student’s t test (n ≥ 100 cells from three independent trials); **, P ≤ 0.01 when also compared with nup116ΔGLFG.