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. 2020 Jun 1;29:0963689720927405. doi: 10.1177/0963689720927405

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 1. — The expression of LINC00460 was upregulated in HNSCC tissue samples and was correlated with poor prognosis in HNSCC. (A) Relative LINC00460 expression in HNSCC from TCGA database. In all, 519 HNSCC tumor tissues and 44 normal tissues, respectively. (B) Kaplan–Meier survival analysis of overall survival according to LINC00460 expression in HNSCC from TCGA database. (C) LINC00460 expression in 15 pair samples of HNSCC and adjacent normal tissues. (D) Relative levels of LINC00460 in HOEC, a normal human oral mucosa cell line and three types of HNSCC cells, CAL-27, WSU-HN4, and WSU-HN6, were determined by qRT-PCR. (E, F) Subcellular location of LINC00460 in CAL-27, and WSU-HN4 cells. U6 and GAPDH acted as nucleus and cytoplasm marker, respectively. Data are presented as the mean ± SD, analyzed using Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. GAPDH: glyceraldehyde 3-phosphate dehydrogenase; HNSCC: head and neck squamous cell carcinoma; qRT-PCR: quantitative real-time polymerase chain reaction.