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. 2020 Jun 1;29:0963689720927405. doi: 10.1177/0963689720927405

Fig. 4.

Fig. 4.

Overexpression of miR-4443 inhibit cell growth, migration, and induced apoptosis in vitro. (A) Relative levels of miR-4443 on HOEC, a normal human oral mucosa cell line and two types of HNSCC cells, CAL-27 and WSU-HN4, were determined by qRT-PCR. (B) Relative miR-4443 expression in CAL-27 and WSU-HN4 cells after transfection with miR-4443 mimics. (C, D) CCK-8 proliferation assays in CAL-27 and WSU-HN4 cells after transfection with miR-4443 mimics or mi-NC. (E, F, G) Transwell migration assay in CAL-27 and WSU-HN4 cells after transfection with miR-4443 mimics or mi-NC. Magnification: ×100. (H, I) Apoptosis rate in CAL-27 and WSU-HN4 cells after transfection with miR-4443 mimics or mi-NC by flow cytometry. Data are presented as the mean ± SD, analyzed using Student’s t-test or analysis of variance. *P < 0.05, **P < 0.01, ***P < 0.001. HNSCC: head and neck squamous cell carcinoma; qRT-PCR: quantitative real-time polymerase chain reaction; SD: standard deviation.