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. 2020 Jun 24;29:0963689720934418. doi: 10.1177/0963689720934418

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — TCF-4 influences hADMSC adipogenic differentiation. (A) The expression of TCF7L2 was assessed by qPCR in cells transfected with the EX-TCF7L2 or siTCF7L2 vectors, resulting in significant increases and decreases in the expression of this gene, respectively, relative to cells transfected with control constructs. (B) Cells overexpressing TCF7L2 were evaluated via qPCR, revealing significant decreases in the expression of C/EBP, PPARγ, and aP2, and significant increases in the expression of TCF7L2 and β-catenin relative to cells transfected with the control construct. (C) Cells in which TCF7L2 had been knocked down were evaluated via qPCR, revealing significant increases in the expression of C/EBP, PPARγ, and aP2, and significant decreases in the expression of TCF7L2 and β-catenin relative to cells transfected with the control siRNA construct. *P < 0.05. GAPDH: glyceraldehyde 3-phosphate dehydrogenase; hADMSC: human adipose-derived mesenchymal stem cell; PPARγ: peroxisome proliferator-activated receptor γ; qPCR: quantitative polymerase chain reaction; TCF-4: T cell factor 4.