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. 2020 Jun 24;29:0963689720934418. doi: 10.1177/0963689720934418

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 8. — miR-26b-5p regulates TCF-4 so as to influence hADMSCs’ adipogenic differentiation. (A) qPCR revealed that when hADMSCs were co-transfected with an miR-26b-5p mimic and EX-TCF7L2, they exhibited significant increases in the expression of C/EBP, PPARγ, and aP2, and significant decreases in the expression of TCF7L2 and β-catenin relative to cells co-transfected with miR-26b-5p and EX-Ctrl constructs. (B) Western blotting revealed that when hADMSCs were co-transfected with an miR-26b-5p mimic and EX-TCF7L2, they exhibited significant increases in C/EBP, PPARγ, and aP2 levels, and significant decreases in levels of TCF-4, cytoplasmic β-catenin, and nuclear β-catenin. (C) Nuclear β-catenin staining intensity was reduced in cells transfected with an miR-26b-5p mimic and EX-TCF7L2. (D) qPCR revealed that when hADMSCs were co-transfected with an miR-26b-5p inhibitor and siTCF7L2 they exhibited significant decreases in the expression of C/EBP, PPARγ, and aP2, and significant increases in the expression of TCF7L2 and β-catenin relative to cells co-transfected with an miR-26b-5p inhibitor and siR-Ctrl constructs. (E) Western blotting revealed that when hADMSCs were co-transfected with an miR-26b-5p inhibitor and siTCF7L2 they exhibited significant decreases in C/EBP, PPARγ, and aP2 levels, as well as significant increases in levels of TCF-4, cytoplasmic β-catenin, and nuclear β-catenin. (F) hADMSCs transfected with miR-26b-5p inhibitor and siTCF7L2 exhibited significant decreases nuclear β-catenin fluorescence intensity. *P < 0.05. GAPDH: glyceraldehyde 3-phosphate dehydrogenase; hADMSC: human adipose-derived mesenchymal stem cell; PPARγ: peroxisome proliferator-activated receptor γ; qPCR: quantitative polymerase chain reaction; TCF-4: T cell factor 4.