Table 1.

Plating data for spheroids generation.

Conditions	IEQ	Petri	Drops	Molds	Kugel
Number of islets or cells	300 IEQ	375 000 cells	375 000 cells	375 000 cells	375 000 cells
Number of spheroids	—	∼1 500	∼1 500	∼1 500	∼1 500
Substrate type	Petri 35 mm	Petri 35 mm	Petri 150 mm	256-well agarose molds in 12-well plate	Engineered microwells in Kugelmeier plate
Number of substrate	1 petri	1 petri	11 petri	6 molds	2 microwells of the 24-well plate
Culture medium volume	2.5–3 ml	2.5–3 ml	45 ml	12.9 ml	4 ml
Time for seeding	30"	30"	120’	2.5’ seeding 30’ sedimentation 2.5’ adding medium	30"
Support devices

Table 1 describes the amount of cells, materials, time needed to plate the cells in the five conditions, and the support device used for each condition.

^a Picture from https://www.kugelmeiers.com/