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. 2020 Jul 22;29:0963689720926147. doi: 10.1177/0963689720926147

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — CircCNST facilitated the proliferation and colony formation of OS cells. (A) Schematic representation of target sequences of the shRNA specific to the back-splicing junction of circCNST. (B) qRT-PCR analysis of the expression levels of circCNST in different OS cell lines. (C) qRT-PCR analysis of the transfection efficiency of circCNST plasmid in SW-1353 and MG-63 cell lines or sh-circCNST in HOS and U-2OS cell lines. (D, E) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis of the cell viability after the transfection with circCNST plasmid in SW-1353 and MG-63 cell lines or sh-circCNST in HOS and U-2OS cell lines. (F, G) Colony formation analysis of the cell colony number after the transfection with circCNST plasmid in SW-1353 and MG-63 cell lines or sh-circCNST in HOS and U-2OS cell lines. Data are the means ± standard error of the mean of three experiments. *P < 0.05, **P < 0.01. CNST: consortin; OS: osteosarcoma; qRT-PCR: quantitative real-time polymerase chain reaction.