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. 2020 Jul 22;29:0963689720926147. doi: 10.1177/0963689720926147

Figure 4.

Figure 4.

CircCNST acted as a sponge of miR-421 in OS cells. (A) Schematic representation of the binding sites of miR-421 with circCNST, and fluorescence in situ hybridization analysis of the colocalization of circCNST with miR-421 in MG-63 cells. (B) Pearson’s correlation analysis of the correlation of circCNST expression with miR-421 in OS tissues. (C) Luciferase activity of WT or Mut circCNST after the transfection with miR-421 mimic or miR-NC in SW-1353 and MG-63 cell lines. (D) qRT-PCR analysis of the expression levels of miR-421 after the transfection with circCNST plasmid in SW-1353 and MG-63 cell lines or sh-circCNST in HOS and U-2OS cell lines. (E) RNA immunoprecipitation and qRT-PCR analysis of the enrichment of circCNST and miR-421 pulled down from the Ago2 protein in SW-1353 and MG-63 cell lines. (F) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide analysis of the cell viability after the co-transfection with circCNST plasmid and (or) miR-421 mimic in SW-1353 and MG-63 cell lines. Data are the means ± standard error of the mean of three experiments. *P < 0.05. CNST: consortin; OS: osteosarcoma; qRT-PCR: quantitative real-time polymerase chain reaction.