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. 2020 Sep 7;9(9):2041. doi: 10.3390/cells9092041

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Fluorescence (Hoechst 33,342—blue, nuclei and BODIPY 493/503—green, LBs) and coherent anti-Stokes Raman scattering images (CARS) of human eosinophils and neutrophils. (B) Multi-parametric morphological analysis of nuclei (upper) and LBs (lower) in fluorescence images. (C) A graph showing the number of LBs per cell, calculated from fluorescence images. Values are given as mean ± standard deviation and are shown in box plots: mean (horizontal line), SE (box), minimal and maximal values (whiskers).