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EVs derived from cancer cell cultures using ABA and ACA. EVs in the culture media with BxPC3, Capan-1, MCF7, HCT116, and A549 cells were quantified with ExoCounter. (A) Total EV detection using anti-CD63 Ab-coated disc and anti-CD9 Ab-conjugated beads. (B) Lectin-binding EV detection using the ABA- or ACA-coated disc and anti-CD9 Ab-conjugated beads. (C) Percentage of the ABA- or ACA-positive EVs normalized to CD63/CD9-positive EVs. Data are presented as means ± SD (n = 3).
