Table 1.
Primers used for the detection of genes encoding beta-lactamases (bla) and mobile colistin resistance (mcr).
| Primer | 5′–3′ |
|---|---|
| KPC-1 | GCCGTGCAATACAGTGATAAC |
| KPC-2 | GAACGTGGTATCGCCGATAG |
| KPC-probe | TexRed-CCGCCGCCAATTTGTTGCTGAAGG-BHQ2 |
| OXA48-1 | GTTGGAATGCTCACTTTACTGAA |
| OXA48-2 | TTCGCCCGTTTAAGATTATTGG |
| OXA48-probe | FAM-ATTCTCATTCCAGAGCACAACTACGCC-BHQ1 |
| GES-1 | CTCTGTGAGTCGGCTAGACC |
| GES-2 | CGATCAGCCACCTCTCAATG |
| GES-probe | HEX-ACACCTGGCGACCTCAGAGATACAACT-BHQ1 |
| VIM-1 | GTTTGGTYGCATATCGCAAC |
| VIM-2 | CTTYTCAATCTCCGCGAGAAG |
| VIM-probe1 | FAM-AGCAACTCATCRCCATCACGGACAATG-BHQ1 |
| VIM-probe2 | FAM-AACTCGGTGACACGGTGTACTCGTCT-BHQ1 |
| NDM-1 | CGACTTATGCCAATGCGTTG |
| NDM-2 | CGGGGTAAAATACCTTGAGC |
| NDM-probe | HEX-AGCCTGACTTTCGCCGCCAATG-BHQ1 |
| OXA23-1 | CCTGATCGGATTGGAGAACC |
| OXA23-2 | GTTCCTGATAGACTGGGACTG |
| OXA23-probe | FAM-TGGCTTCTCCTAGTGTCATGTCTT-BHQ1 |
| OXA58-1 | GTTGGTATGTGGGTTTTGTTG |
| OXA58-2 | CGTAGAGCAATATCATCACCAG |
| OXA58-probe | Cy5-TGCCACCACTTGCCCATCTGCC-BBQ®-650 |
| CTXM-1-1 | AATCTGACGCTGGGTAAAGC |
| CTXM-1-2 | GATATCGTTGGTGGTGCCATA |
| CTXM-1-Probe | FAM-CCTGAATGCTCGCTGCACCGG-BHQ1 |
| CTXM-9-1 | CCGATCTGGTTAACTACAATCC |
| CTXM-9-2 | GCTGGGCAATCAATTTGTTC |
| CTXM-9-probe | TexRed-CAACGGCACAATGACGCTGGC-BHQ2 |
| CMY2-1 | GATGCAGGAGCAGGCTATTC |
| CMY2-2 | AACACGCCGTTAAACGTCTTAC |
| CMY2-probe | FAM-CCAATAACCACCCAGTCACGCAG-BHQ1 |
| FOX-1 | GTTCGAGATTGGCTCGGTCA |
| FOX-2 | CACTGTAGGTGGCAAGCTCG |
| FOX-probe | HEX-TGGCTCACCTTGTCATCCAGC-BHQ1 |
| ACT_MIR-1 | ACTGGCAGCCGCAGTGGAAG |
| ACT_MIR-2 | ACGTTAATCCASGTATGGTCCAGC |
| ACT_MIR-probe | FAM-AGACCCGCGTCGTYATGGCCTG-BHQ1 |
| DHA-1 | GGCGATATGCGTCTGTATGC |
| DHA-2 | GTCAGCAACTGCTCATACGG |
| DHA-probe | TexRed-CCTGTTTGGTGCTCTGACCGC-BHQ2 |
| mcr-1-1 | ATGGCACGGTCTATGATACG |
| mcr-1-2 | CACACCCAAACCAATGATACG |
| mcr-1-probe | FAM-ACCGACCAAGCCGAGACCAAGGA-BHQ1 |
| mcr-2-1 | GCCAACAGACACCATCTATC |
| mcr-2-2 | TAGCCATTGAACTGCACATG |
| mcr-2-probe | HEX-ACCACCAAGCCGAGCGAGCG-BHQ1 |