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. 2020 Jul 1;29:0963689720938023. doi: 10.1177/0963689720938023

Figure 2.

Figure 2.

Effect of linc02381 overexpression and linc02381 knockdown on RA-FLS proliferation and invasion. Blank vector, pcDNA-linc02381 (linc02381, 1 μg/ml), NC siRNA, or linc02381 siRNA (20 or 50 nM) vector was transfected into FLSs. (A) The overexpression efficiency of pcDNA-linc02381 expression in FLSs was confirmed by RT-qPCR. (B–E) Cell proliferation was detected by CCK-8 assay or EdU assay, and cell invasion was detected by Transwell assay after FLSs were transfected with pcDNA3.1-linc02381. (F) The expression of linc02381 was examined by RT-qPCR in FLSs transfected with 20 or 50 nM linc02381 siRNA. (G–I) Cell proliferation and invasion of FLSs following transfection with 20 nM linc02381 siRNA or 50 nM linc02381 siRNA. (J) Cell apoptosis was detected by flow cytometry assay in FLSs transfected with 20 or 50 nM linc02381 siRNA. *P < 0.05 or **P < 0.01. Three independent experiments were carried out, respectively. CCK-8: Cell Counting Kit-8; NC: negative control; RA: rheumatoid arthritis; RA-FLSs: RA fibroblast-like synoviocytes; RT-qPCR: quantitative real-time polymerase chain reaction; siRNA: small interfering RNA.