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. 2020 Jul 1;29:0963689720938023. doi: 10.1177/0963689720938023

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Prediction and confirmation of interaction between linc02381 and miR-590-5p. (A) Details of the potential binding site of linc02381 and miR-590-5p in wild-type linc02381 and the mutant linc02381 sequences. (B) Verification of the relationship between linc02381 and miR-590-5p by the luciferase gene reporter assay. (C) Verification of the relationship between linc02381 and miR-590-5p by RNA pull-down assay based on the biotinylated miR-590-5p. (D) The effect of linc02381 overexpression and knockdown on the expression of miR-590-5p, respectively. (E) NC mimic or miR-590-5p mimic was transfected into RA-FLSs, and the expression of linc02381 was detected by RT-qPCR. **P < 0.01. Three independent experiments were carried out, respectively. NC: negative control; RA: rheumatoid arthritis; RA-FLSs: RA fibroblast-like synoviocytes; RT-qPCR: quantitative real-time polymerase chain reaction.