Figure 2.

Growth kinetic in macrophages of the two ASFV strains collected from illegal free-ranging pigs. moMΦ were infected with the strains under study (103917/14 or 55234/18) or the virulent 26544/OG10 ASFV strains using a multiplicity of infection (MOI) of 0.01. At 0, 24, 48, and 72 h pi, duplicate samples were collected, and infectious viral progeny in culture supernatants were assessed by titration (TCID₅₀/mL). The sensitivity of this assay was 1.8 log₁₀ 50% haemadsorbing doses/mL (TCID₅₀/mL). The mean values and the standard deviation from three independent experiments utilizing different animals are shown. At each time-point, values of 103917/14 and 55234/18 and 26544/OG10 viral titres were compared using the analysis of variance (ANOVA) for repeated measures.