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. 2020 May 28;29:0963689720929987. doi: 10.1177/0963689720929987

Fig. 4.

Fig. 4.

Resveratrol reverses the inhibitory effects on apoptosis in pancreatic cancer cells induced by CMs from hypoxia-activated PSCs. CM were from PSCs activated by hypoxia. (A, B) Panc-1 cells were cultured under normoxic conditions with CM from PSCs treated with or without 50 μM resveratrol or si-HIF-1α for 24 h. Next, Panc-1 cell apoptosis was detected by flow cytometry. (C) Subconfluent Panc-1 cells were treated under normoxic conditions with CM from PSCs treated with or without either 50 μM resveratrol or si-HIF-1α for 24 h. Next, the cells were lysed, and the protein levels of the apoptosis markers BCL2 and cleaved caspase-3 in Panc-1 cells were analyzed by western blotting. #P < 0.05; ##P < 0.01comparing the CM group with the control group. *P < 0.05; **P < 0.01comparing the CM+RSV or CM+si-HIF-1α group with the CM group. All data represent at least three independent experiments. CM: conditioned medium; HIF-1α: hypoxia-inducible factor 1; PSC: pancreatic stellate cell; RSV: resveratrol.