Skip to main content
. 2020 Apr 9;10(9):1619–1633. doi: 10.1016/j.apsb.2020.03.006

Figure 4.

Figure 4

ICS Ⅱ facilitates NLRP3-dependent ASC oligomerization, but has no impact on NLRP3–NLRP3 interaction and intracellular potassium. (A) Western blot analysis of ASC oligomerization in lysates from BMDMs stimulated with LPS and ATP (1 h) and treated with various doses of ICS Ⅱ. (B) Western blot analysis of ASC oligomerization in lysates from LPS-primed BMDMs treated with ICS Ⅱ (5 μmol/L) before ATP (1 h), MSU (6 h), poly(dA:dT) (6 h), Lfn-Flic (6 h) stimulation or Pam3CSK4-primed BMDMs treated with ICS Ⅱ (5 μmol/L) and then transfected with LPS (6 h). (C) Western blots of cell lysates and flag-immunoprecipitation samples from HEK-293T cells transfected with Flag-NLRP3 and Myc-NLRP3. (D) Qualification of potassium efflux in LPS-primed BMDMs treated with various doses of ICS Ⅱ and then stimulated with ATP (1 h). Data are means ± SEM (n = 3).