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. 2020 Apr 27;10(9):1741–1753. doi: 10.1016/j.apsb.2020.04.010

Figure 2.

Figure 2

(A) Molecular structures of cationic core PEI-SS-VES and multifunctional outer layer HA-PEG-RGD. (B) Formation of pGDTA@PVHRs NP, which were constituted of pGDTA, PEI-SS-VES and HA-PEG-RGD (schematic). (C) Gel electrophoresis assay of pGDTA@PVHRs NP at different N/P ratio. (D) TEM image of pGDTA@PVHRs NP. (E) Particle size and zeta potential changes of pDNA@PVHRs NP at pH 7.4. Data were presented as mean ± SD (n = 3). (F) Particle size change of PVHRs NP incubated in 1 mg/mL HAase and/or 10 mmol/L GSH solution (pH 7.4) for 30 min. (G) Particle size and zeta potential changes of PVHRs NP incubated in 1 mg/mL HAase at pH 7.4 or pH 5.5. Data were presented as mean ± SD (n = 3). (H) In vitro gene release of PVs micelles and PVHRs NP loading YOYO-1 labeled pGDTA in the PBS (pH 7.4) with or without 10 mmol/L GSH. Data were presented as mean ± SD (n = 3). HAase- and redox-responsive release profile of pGDTA from PVHRs NP incubating with 1 mg/mL HAase and/or 10 mmol/L GSH at (I) pH 7.4 and (J) pH 5.5. Data were presented as mean ± SD (n = 3).