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. 2019 Dec 18;10(9):1694–1708. doi: 10.1016/j.apsb.2019.12.011

Figure 6.

Figure 6

Effect of Rd on tumor vessels in Balb/c mice harboring LoVo xenografts. Mice were intraperitoneally injected with SMI, Rd, Rb1, Rg1, or vehicle for 15 days. Maximum intensity projection of the Z-positions with CD31 staining of tumor vascular endothelial cells (n = 3–4; thickness = 100 μm; scale bar = 100 μm). (B) and (C) Statistical quantification of CD31+ microvessels (<10 μm in vessel diameter, B) and vessel branches (C) in (A). (D) Double staining of CD31 (red) and α-SMA (green) in tumors revealing pericyte coverage around tumor vessels (n = 4; thickness = 12 μm; scale bar = 100 μm). (E) Statistical quantification of pericyte coverage based on calculations of the α-SMA+ fractions in (D). (F) and (G) The secretion level of VEGF (F) and FGF (G) in tumors (n = 6–7). Data were expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle group.