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Comparison of absolute quantitation of total HA by SDS-PAGE and by label-free LC-MS/MS.
| U3 | U4 | VG | |
|---|---|---|---|
| HA concentration by SDS PAGE, μg/mL | 62.30 | 88.91 | 68.13 |
| HA concentration by LC-MS/MS, μg/mL | 58.12 | 82.23 | 64.84 |
| Relative difference, % | 6.72 | 7.51 | 4.83 |
Note: Relative difference (%) is the ratio of the difference between total HA concentrations quantified with SDS-PAGE and with LC-MS/MS to total HA concentration quantified with SDS-PAGE.
