Table 1.
Primers used in this study. The restriction site in each primer is underlined.
| Primer Name | Sequence (5′–3′) |
|---|---|
| (a) Primers used to generate and confirm ∆spnAi and ∆s5nAi | |
| SpnAi_FR1.fw | GCCGGATCCGCAGGCCAATTATCTCTTAG |
| SpnAi_FR1.rv | CTAAGCTTATTGTAACAGAAACATCAGTC |
| SpnAi_FR2.fw | CATGCCATGGCACGCCGGAGAAACTTCTG |
| SpnAi_FR2.rv | CCCCCCGGGAACGGACCACGATGCCAC |
| S5nAi_FR1.fw | GCCGCTAGCGAAAACCATCAAGGCTTCAACG |
| S5nAi_FR1.rv | GGCGAGCTCCCGTGGAAATCGTTGACACC |
| S5nAi_FR2.fw | CATGCCATGGGCCAAAACAAGCACAATGG |
| S5nAi_FR2.rv | GCTGAATTCGCTTAATGAAGAGCATGCG |
| aad9.fw | CCTTATTGGTACTTACATGTTTG |
| SpnAi_DP.rv | GACACTGAACAGGCCTTGGCTG |
| S5nAi_DP.rv | GTCGATTAAGGCTGATTTAGCC |
| (b) Primers used to generate ∆spnAi:spnAi and ∆s5nAi:s5nAi | |
| SpnAi_FL_ORF.fw | CAGGATCCTAAAGGAGTTTTTATGTTAAAC |
| SpnAi_FL_ORF.rv | CGGAATTCTTAGTTTTTTTGACCTTTACG |
| S5nAi_FL_ORF.fw | CGGGATCCATTAGGAGTTTATATGAAAAAGC |
| S5nAi_FL_ORF.rv | CGGAATTCTTAGTTTTCTTCTTTTTTCTTGC |