Figure 5.

Immunofluorescence staining of FCV F9- and human coronavirus (HCoV) 229E-infected cells. CRFK and L132 cells were infected with FCV F9 and HCoV 229E, respectively, for 24 h and treated with DMSO-solubilized extract and water-solubilized extract (16 µg/mL) prior to fixation with methanol. The infected cells were then incubated with anti-FCV F9 and anti-HCoV 229E antibodies, then stained with goat anti-mouse FITC secondary antibody and treated with DAPI as counterstain. The photos were obtained at 40× magnification power. Scale bars: 20 µm.