Table 2.
List of primary and secondary antibodies used for immunoblotting.
| Antibody | Source | Dilution | Cat. Number | Manufacturer |
|---|---|---|---|---|
| p-ATR (Ser428) | Rb/pcl | 1:1000 | 2853 | Cell Signaling |
| p-BRCA1 (Ser1524) | Rb/pcl | 1:1000 | 9009 | Cell Signaling |
| p-CHK1 (Ser345) (133D3) | Rb/mcl | 1:1000 | 2348 | Cell Signaling |
| CHK1 (FL-476) | Rb/pcl | 1:1000 | sc-7898 | Santa Cruz Biotechnology |
| p-CHK2 (Thr68)(C13C1) | Rb/mcl | 1:1000 | 2197 | Cell Signaling |
| CHK2 (DCS-270) | Ms/mcl | 1:1000 | sc-56296 | Santa Cruz Biotechnology |
| p21 Waf1/Cip1 | Ms/mcl | 1:1000 | 05-345 | Merck Millipore |
| p-histone H2AX (Ser139) | Rb/mcl | 1:1000 | sc-101696 | Cell Signaling |
| CDC27 (AF3.1) | Ms/mcl | 1:1000 | sc-9972 | Santa Cruz Biotechnology |
| CDC20 | Rb/pcl | 1:1000 | ab26483 | Abcam |
| AURKB/AIM1 | Rb/pcl | 1:1000 | 3094 | Cell Signaling |
| PLK1 | Ms/mcl | 1:500 | sc-17783 | Santa Cruz Biotechnology |
| WEE1 | MS/mcl | 1:500 | sc-5285 | Santa Cruz Biotechnology |
| BUB3 | Ms/mcl | 1:1000 | sc-376506 | Santa Cruz Biotechnology |
| MAD2L1 (D8A7) | Rb/mcl | 1:1000 | 4636 | Cell Signaling |
| pVHL | Ms/mcl | 1:200 | 556347 | BD Pharmingen |
| Actin (C-11) | G/pcl | 1:1000 | sc-1615 | Santa Cruz Biotechnology |
| Kv10.1 | Rb/pcl | 1:1500 | 9391 | |
| Normal Mouse IgG | Ms | Isotype control | sc-2025 | Santa Cruz Biotechnology |
| Normal Rabbit IgG | Rb | Isotype control | sc-3888 | Santa Cruz Biotechnology |
| ECL Anti-Rabbit IgG, HRP Linked | D/pcl | 1:10,000 | NA934 | GE Healthcare |
| ECL Anti-Mouse IgG, HRP Linked | Sh/pcl | 1:10,000 | NA931 | GE Healthcare |
| Anti-Goat IgG (H + L)-HRP Conjugate | Rb/pcl | 1:10,000 | 172-1034 | BIO-RAD |
MS—mouse; Rb—rabbit; G—goat; Sh—sheep; mcl—monoclonal; pcl—polyclonal.