Figure 4.

Tubuline cytoskeleton in cell cycle interphase of root cells from WT (a–c) and transgenic tomato (d–f) plants grown without, and with the addition of NaCl and Na₂SO₄. Nuclei were stained with DAPI (blue); microtubules were detected using antibodies to α-tubuline (green). While the bundles of microtubules are located in the cortical cytoplasm, microtubules are not visible in the perinuclear region in the interphase cells. Tomato root cells have obvious multiple lesions in the location of microtubules under the NaCl, Na₂SO₄. Cells form multiple chaotically arranged bundles and do not maintain peripheral position, which indicates violations in the process of cytoskeleton transformation, as a highly dynamic structure.