Figure 3.

RNA-seq analysis of differentially expressed genes among hyperglycemic EVs. (A) Venn diagram representation of differential genes (only FC ≥ 2 and FDR < 5% were considered) of total RNAs, mRNAs, and non-coding RNAs: CBSC- (yellow), CPC- (green), and EPC- (purple) expressed genes, and common genes (grey) are shown. A total 324 genes (241 genes of mRNAs, 21 of ncRNAs, and 16 miRNAs) were presented in all stem cell types. (B) Heat maps show differentially expressed mRNAs. EV RNA-seq data were analyzed using DESeq2 package. Red indicates higher expression and blue indicates lower expression (n = 2 mice/group).