Figure 1.

Design, cloning and production of ChAdOx1 and MVA viral vectored vaccines expressing P. cynomolgi (Pc) circumsporozoite protein (PcCSP) and Thrombospondin Related Adhesive Protein (PcTRAP). (A–E). (A) Agarose gel showing restriction fragment length polymorphism (RFLP) of the initial plasmids containing the synthetic genes of PcCSP and PcTRAP genes (B) Agarose gel showing PcCSP and PcTRAP bands upon enzyme restriction of from Shuttle Vectors and final virus plasmids. (C–E) Each cassette was cloned into a Shuttle vector and subsequently into both, adenoviral ChAdOx1 and Modified Vaccinia Ankara (MVA) vectors.