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. 2020 Jul 6;8(3):363. doi: 10.3390/vaccines8030363

Figure 2.

Figure 2

Design and cloning of PcCSP and PcTRAP transgenes for protein expression. (A) Agarose (1%) gel showing the PcCSP and PcTRAP PCR products following the restriction with AgeI and KpnI enzymes. (B) Agarose (1%) gel showing a single or double digestion with AgeI and KpnI to confirm the insertion of the PcCSP and PcTRAP into the PhLSec plasmid. (C) SDS-PAGE coomassie-stained gel (left) and Western blot (WB) with an anti-His monoclonal antibody (right) for detection of PcCSP and PcTRAP proteins upon transfection of HEK293 cells with PhLSec plasmids expressing either transgene.