Figure 3.
Cellular immune responses elicited by ChAdOx1 and MVA vaccines. C57Bl/6 mice (n = 3 per group) were injected with either ChAdOx1 PcCSP or ChAdOx1 PcTRAP at a concentration of 1 × 108 IU/mouse, and MVA PcCSP and MVA PcTRAP at concentrations of 1 × 106 PFU/mouse. A heterologous Prime-Boost using ChAdOx1-MVA vaccination regimen was also tested. Peripheral blood mononuclear cells (PBMCs) were cultured with peptide pools spanning the PcCSP and PcTRAP proteins and responses were assessed using an ex vivo IFN-gamma ELISpot. Values represent the spot-forming cells (SFC) per million PBMCs resulting from stimulation with 20-mer overlapping by 10 peptides spanning the whole PcCSP (A) and PcTRAP (B) structural proteins (10 µg/mL). Line shapes represent mice vaccinated with each vaccine. p-values were determined by Kruskal–Wallis test and Dunn’s multiple comparisons test. p > 0.05 (ns), p < 0.05 (*).