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. 2020 Sep 22;12(9):2715. doi: 10.3390/cancers12092715

Figure 6.

Figure 6

MEN1 and OXPHOS expression are increased in breast circulating tumor cells (CTCs). (A) t-SNE profile plots and cell clustering of 93 CTCs from 5 breast cancer patients based on the single cell RT-qPCR expression profiling of 11 OXPHOS genes (NDUFA7, NDUFA11, NDUFA13, NDUFB7, NDUFS7, NDUFS8, NDUFV1, SDHA, SDHB, SDHC, and SDHD). (B) Violin plots of MEN1 or selected MAPs expression, mean expression of 7 glycolytic genes (ALDOA, ALDOC, ENO1, PFKL, PFKP, PGK1, and TPI1) or mean expression of 11 OXPHOS genes (aforementioned) in the five cell clusters. Statistical significance among clusters was carried out using the Duncan multi-range test. (C) Mean expression of 7 glycolytic genes and 11 OXPHOS genes in these 93 breast CTCs or in the TCGA primary breast cancer cohort. (D) Glycolytic and OXPHOS ATP productions of T47D or MCF-7 cells after circulation (n = 6–10 technical replicates). (E,F) Mitochondrial (E) and glycolytic (F) functions of T47D or MCF-7 cells after circulation (n = 5–9 technical-replicate wells). Statistics represented the difference between no circulating control and each treatment. Data are presented as mean ± S.D. An unpaired two-tailed Student’s t-test was used for statistical significance determination. * p < 0.05, ** p < 0.01, and *** p < 0.001.