FIGURE 1.

DHA increased MANF expression, decreased TREM2 expression, attenuated infarct size, and led to neurological protection 24 h after MCAo. Rats were treated with DHA or saline at 3 h after onset of MCAo and sacrificed 24 h later. A, Experimental design for series 1 and 2; B, Total neurological score (normal score = 0, maximal deficit = 12) in DHA and saline‐treated groups; C, Computer‐generated MosaiX‐processed images of Nissl‐stained brain sections and cortical, subcortical infarct volume and brain swelling; D, E, Western blot analysis of MANF/GAPDH and TREM2/GAPDH expression in ipsilateral cortex and subcortex. Value is mean ± SEM N = 6/group, *P < .05, DHA vs saline, Student t test