FIGURE 2.

DHA upregulated neuron and astrocyte MANF 24 h after MCAo. Representative images of A, MANF/NeuN⁺ (MANF, red; NeuN, green; Hoechst, blue) and B, MANF/GFAP⁺ (MANF, green; GFAP, red; Hoechst, blue) double immunostaining within cortex, SVZ, and DG. Arrows indicate colocalization of MANF/NeuN (A) and MANF/GFAP (B) positive cells. The images were obtained in the peri‐infarct cortex, SVZ, and DG. Scale bar = 20 µm. C, Location diagrams for cell count and quantification of MANF/NeuN⁺ and MANF/GFAP⁺ cells at +1.2 mm and –3.8 mm of bregma levels. Treatment with DHA increased the number of MANF/NeuN⁺ cells in the cortex and DG and MANF/GFAP⁺ cells in SVZ, cortex, and DG 24h after MCAo. n = 6‐8/group; *P < .05, DHA vs saline; repeated‐measures ANOVA followed by Bonferroni tests. C, peri‐infarct cortex; SVZ, subventricular zone; DG, dentate gyrus; CA1 and CA3, regions of hippocampus