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Methodological approach of the present study. Maize seeds were germinated in vitro; the second leaves of the seedlings were used to obtain the protoplasts. The protoplasts were exposed to the CRISPR-Cas9 ribonucleoproteins complex (RNP), and after 24 h, the DNA was extracted from the samples. PCR fragments were amplified and sequenced. Maize image was taken from the free repository from pexel.com.
