Figure 3.

Microscope photographs of maize protoplast cells transfected with polyethylene glycol (PEG)-mediated CRSIPR-Cas9 RNP. The RNP containing the ATTO550-conjugated trans-activating crispr RNA (tracrRNA) was transfected into maize protoplasts and cells were monitored under fluorescent microscopy to check the transfection efficiency. Comparing to the cells without CRISPR-Cas9 delivery (a—control sample), the red fluorescent signal was detected in CRISPR-Cas9 treated samples (b and c—right). Photographs were taken with white light (left side) and fluorescent light (right side). Blue arrows indicate the internalization of the RNP complex. Scale bars: 100 μm.