Table 1.

List of primers and crispr-RNAs (crRNAs) used for the amplification and mutation of the inositol phosphate kinase gene (IPK) target locus in maize via CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9.

Primer	Sequence (5′–3′)	Amplicon Size (bp)
ZmIPK-F	GAAGAAGCAGCAGAGCTTCA	876
ZmIPK-R	CAGAAGAAATCCGTGAGGACAG
crRNA	Sequence (5′–3′)	Cleavage Fragments (bp)
crRNA1	AGCTCGACCACGCCGCCGAC	279 | 597
crRNA2 *	GGGATCCGTCTCCTTCTCCC	617 | 259
crRNA3 *	ATCTTCAAGGTCTACGTCGT	525 | 351
crRNA4 *	CAGGAGTTCGTCAACCATGG	498 |378
crRNA5	ACAAGCTCTACGGAGACGAC	141 | 735

Note: * Selected crRNAs used for protoplasts transfection.