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. 2020 Sep 12;8(9):1409. doi: 10.3390/microorganisms8091409

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The living bacterial proportion in the BPI C. acnes strain biofilms and comparison with the commensal strain biofilms. Repartition of Syto9 (green bars) and PI (red bars) staining within biofilm formed by the C. acnes strains isolated from BPIs before (a) and after internalization (b) by osteoblast-like cells; (c) comparison of commensal and BPI strains: live dead proportion quantification within biofilm of C. acnes commensal “C” strains and isolated from BPIs. Images acquisition by fluorescent microscopy and calculation by Image J software.