Figure 3.

Myogenesis (a) and neurogenesis (b) of P. excavatus. (a) Myogenesis was visualized by phalloidin staining (red) in embryonic stages of P. excavatus. Arrows and arrowheads indicate longitudinal muscles (along the anterior–posterior axis) and circular muscles (perpendicular to the anterior–posterior axis), respectively. Scale bar, 200 μm. (b) Nervous system development of P. excavatus visualized by immunohistochemistry using anti-acetylated α-tubulin (green). Abbreviations: br, brain; cc, circumesophageal connective; nb, nephroblast; ne, nephridium; pn, palp nerves; sn, segmental nerves; vnc, ventral nerve cord. Scale bar, 200 μm.