Table 2.
Identification of bacterial proteins in mouse skin infected by intradermal inoculation of different Borrelia burgdorferi strains, or via infectious tick bite. Proteins were identified using Mascot algorithm. Numbers of identified peptides are given.
| Protein Name | Accession Number (Borrelia burgdorferi B31) |
Accession Number (Borrelia burgdorferi Pko) |
Infection via Syringe Inoculation Borrelia burgdorferi Strain (Spirochete Density Flagellin/104gapdh) |
Infection via Tick Bite (Spirochete Density Flagellin/104gapdh) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 297c4 (192) |
297c4 (106) |
297n (346) |
IBS19 (85) |
IBS19 (136) |
IBS19 (166) |
BL642 (157) |
BL642 (227) |
BL642 (268) |
B. Afzelii NE4049 (66) |
Field-Collected Ticks (534) |
|||
| Flagellin | gi|120230 | gi|111114970 | 7 | 8 | 7 | 2 | 2 | 3 | 3 | 2 | 4 | 8 | |
| Osp C | gi|3914248 | gi|111074137 | 3 | 2 | 3 | 1 | 2 | 2 | 2 | 1 | 1 | 4 | 1 |
| GAPDH | gi|238828321 gi|3915702 | 1 | 1 | 1 | 1 | 1 | |||||||
| Glycosaminoglycan binding Protein (Bgp) | gi|15594933 | 1 | 1 | 1 | |||||||||
| GroEL | gi|229553917 | gi|123145654 | 1 | 1 | 2 | ||||||||
| p66 | gi|15594948 | 1 | |||||||||||
| Fructose-bisphosphate aldolase | gi|6686370 | gi|111115272 | 1 | ||||||||||
| DbpA | gi|17373807 | 1 | |||||||||||
| DbpB | gi|327507700 | 2 | |||||||||||
| Elongation factor 4 | gi|15594434 gi|6016495 | 1 | |||||||||||
| Elongation factor Tu | gi|1706598 | gi|123341337 | 1 | 1 | 1 | 2 | |||||||
| Enolase | gi|3913583 | 1 | |||||||||||
| Lipoprotein BbA36 | gi|365823350 | 2 | |||||||||||
| L-lactate dehydrogenase | gi|15594433 gi|17367476 | 1 | |||||||||||
| hypothetical protein BB_0363 | gi|15594708 | 1 | |||||||||||
| hypothetical protein BB_0563 | gi|365992369 | 1 | 1 | ||||||||||
| hypothetical protein BB_F14 | gi|365823340 | 1 | 1 | ||||||||||
| hypothetical protein BB_J48 | gi|364556751 | 1 | |||||||||||
| hypothetical protein BAPKO_4515 | gi|117621815 | 1 | |||||||||||
| hypothetical protein BAPKO_0028 | gi|111114851 | 1 | |||||||||||
| hypothetical protein BAPKO_0593 | gi|111115391 | 1 | |||||||||||
| hypothetical protein BAPKO_2500 | gi|117621647 | 1 | |||||||||||
| 50S ribosomal protein L7/L12 | gi|123046997 | 1 | |||||||||||
| 30S ribosomal protein S16 | gi|123145645 | 1 | 1 | ||||||||||
| Neutrophil activating protein | gi|111115523 | 2 | |||||||||||
| Phosphoglyceromutase | gi|123145651 | 1 | |||||||||||
| Flagellar filament outer layer protein | gi|111115501 | 1 | |||||||||||
| ATP-dependent protease | gi|111115078 | 1 | 1 | ||||||||||
| Periplasmic oligopeptide-binding protein | gi|111115153 | 1 | |||||||||||
| Chemotaxis protein methyltransferase | gi|111114863 | 1 | |||||||||||
| Number of Borrelia proteins | 9 | 6 | 7 | 2 | 2 | 2 | 3 | 6 | 5 | 7 | 15 | ||
| Number of mouse proteins | 1831 | 1791 | 1824 | 1486 | 1617 | 1550 | 1702 | 1351 | 1426 | 5916 | 5926 | ||