Skip to main content
. 2020 Aug 30;9(9):2798. doi: 10.3390/jcm9092798

Figure 5.

Figure 5

Number of cells per pellet after 14 and 35 days of cultivation: at day 14 and 35 of cultivation number of cells per pellet was determined using CyQuant® Assay. Number of chondrocytes per pellet at day 14 (left) and day 35 of cultivation (right) differs between used media groups. The following media groups were tested: Negative control (NC), vital cartilage conditioned medium (VC), high hydrostatic pressure treated cartilage conditioned medium (HHPTC) and positive control (PC). The dotted line depicts the initial number of cells. Solid lines within boxes show the median, minimum and maximum, while dots represent the single values. Biological duplicates were used (n = 6). Significance was calculated with Repeated Measures Two-way ANOVA with “time” (14 and 35 days) and “treatment” (NC, VC-CM, HHPTC-CM and PC) as variables. Significance: * p < 0.05 between treatments and # p < 0.05 comparison between day 14 and 35.