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. 2020 May 20;10(9):1634–1645. doi: 10.1016/j.apsb.2020.05.002

Figure 4.

Figure 4

Il-4 deficient neurons are susceptible to OGD injury and alleviation of the ischemia injury by supplemental IL-4. (A) Cortical neurons were subject to 30 min oxygen-glucose deprivation and 24 h reoxygenation (OGD/R) injury. The representative images for morphological changes of Il-4+/+ and Il-4−/− neurons at 24 h after washout. (B) Increase of the lactate dehydrogenase (LDH) release in LDH assay and (C) decrease of cell viability in Cell Counting Kit-8 (CCK8) assay of Il-4−/− primary cortical neurons subject to OGD/R injury. Data are expressed as the mean ± SEM, n indicates the number of mice in each group, ∗P < 0.05, ∗∗P < 0.01 versus Il-4+/+ group. (D) The representative morphological images of Il-4−/− cortical neurons after adding IL-4 (20 ng/mL) for 7 days and subject to 20 min OGD/24 h R injury. Supplementing IL-4 increased the cell viability of Il-4−/− cortical neurons in the OGD injury group (E), but not in the Sham group (F). Data are expressed as the mean ± SEM. The numbers at the bottom of the bars indicate the number of repeats. ∗∗P < 0.01 versus Il-4+/+ group, paired student t-test. n.s.: no significance.