Figure 5.

Expression of astrocytic markers. The iPSC and BFCN samples were analysed by nCounter (Nanostring) and, after normalisation of total amount of RNA molecules to the housekeeper genes, results are shown as fold change of BFCN cultures over iPSCs. Analysis of the astrocyte markers (A) ALDOC, (B) SLC1A3 (EAAT1), (C) NFIA and (D) S100B. Data are derived from 10 iPSC lines and 10 BFCN differentiated samples from 1 independent differentiation for each line. Individual data are shown. Histogram bars represent mean values and error bars represent S.E.M. Graphs show *** p ≤ 0.001, by Kolmogorov-Smirnov test. BFCN cultures were matured for 4 weeks and immunocytochemistry for general neuronal markers and specific cholinergic neuronal markers was performed. (E–G) Representative confocal pictures of the BFCN cultures at week 4 of BFCN maturation with the nuclear marker Hoechst 33,342 in blue. (E) Astrocyte marker GFAP in green. (F) Dendritic marker MAP2 in red. (G) Merged picture of G and H. Scale bars = 50 μm.