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. 2020 Sep 16;9(9):294. doi: 10.3390/biology9090294

Figure 3.

Figure 3

Mlp124357 possesses a GxxxG motif that is required for the interaction with tonoplasts. (A) Schematic representation of the GxxxG motif in the C-terminus region of Mlp124357. (B) A predicted helical wheel projection of Mlp124357. The glycine residues at positions 76 and 80 are indicated by square boxes. (C) Fluorescence imaging of N. benthamiana cells expressing eGFP fusions of Mlp124357 and Mlp124357 GA mutant at 2 dpi using confocal microscopy of epidermal cells. (D) Morphology of each genotype. Photographs are from 4-week-old soil-grown plants. (E) Leaves of each genotype were infiltrated with a PstDC3000 bacterial suspension at OD 600 = 0.001 and bacterial growth was quantified in colony-forming units (cfu) on day 0 and day 3 after infection. One-way ANOVA (p < 0.05) with Tukey’s test was performed. The asterisk indicates a statistically significant difference between Col-0-eGFP, Mlp124357-eGFP, and Mlp124357GA-eGFP. Five replicates were used for each genotype. (F) Each genotype was spray inoculated with H. arabidopsidis Noco2 spores (20,000 conidiospores/mL) and the number of conidiospores was quantified 7 days after inoculation. Statistical significance was evaluated using one-way ANOVA with Tukey’s test (significance set at p < 0.05). The asterisk denotes a significant difference between Col-0-eGFP, Mlp124357-eGFP, and Mlp124357GA-eGFP. FW, fresh weight. Both bacterial and oomycete infection experiments were repeated at least three times and representative data are shown.