Table 1.

Details of the primers designed to validate CRISPR/Cas9 constructs (primer A and D) and genotype the exon-v6 skipping cell models obtained by CRISPR/Cas9 and Phosphorodiamidate Morpholino Oligomers (PMOs) approaches (primer B, C, E and F).

Orientation	In-Text Name	Binding Site	Primer Sequence (5′–3′)	Melting Temperature
Forward	A	pSpCas9(BB)_Bbs I	5′-GGGCCTATTTCCCATGATTCCTT-3′	Tm = 68.5 °C
	B	CD44 Exon 10 (v5)	5′-ATGTAGACAGAAATGGCACCAC-3′	Tm = 62.7 °C
	C	CD44 Intron 10	5′-ATCAGTGGCCTGTTTCCTTG-3′	Tm = 64.0 °C
Reverse	D	pSpCas9(BB)_Bbs I	5′-GACTCGGTGCCACTTTTTCAA-3′	Tm = 66.2 °C
	E	CD44 Exon 12 (v7)	5′-CCATCCTTCTTCCTGCTTGATG-3′	Tm = 66.8 °C
	F	CD44 Intron 11	5′-TTTGGCTCTGTGTGAACTGC-3′	Tm = 64.1 °C