Figure 3.
Expression of 17 G. mellonella growth markers during C. difficile colonisation and phage therapy regimens. The relative expression levels of (A, B) 9 growth, (C) infection and reproduction and (D) cellular and humoral genes were examined during C. difficile colonisation in G. mellonella larvae and when undergoing different phage therapy regimens. G. mellonella larvae were either untreated (1), treated with empty needles (2), 10 µL of either BHI (3), or 105 CFU/mL of CD105LC2 bacterial culture (4). The larvae were also treated with a co-infection of the phages (106 PFU/mL) and bacteria (5), remedially (6) or prophylactically (7), and compared to a group of larvae treated with just the phage cocktail (8). Experiments were repeated three times. Values were normalised using the housekeeping gene 18S rRNA and analysed using GraphPad Prism 8.