Table 1.
Treatment regimens for the controls, prophylactic and remedial phage therapy used in this study.
| Experimental Groups | Treatments | Time (h) | ||||
|---|---|---|---|---|---|---|
| 0 | 2 | 24 | 48 | 72 | ||
| 1 | Control 1—Untreated | - | - | - | - | - |
| 2 | Control 2—Empty needle | N | N | - | - | - |
| 3 | Control 3—BHI | BHI | BHI | - | - | - |
| 4 | Control 4—Bacteria (CD105LC2) | B | BHI | - | - | - |
| 5 | Phage/bacteria co-infection | P+B | BHI | - | - | - |
| 6 | Remedial regimen | B | P | - | - | - |
| 7 | Prophylactic regimen | P | B | - | - | - |
| 8 | Control 5—Phage only | P | BHI | - | - | - |
Insects were treated with either an empty needle (N), 10 µL of either brain heart infusion broth (BHI), 105 CFU/larva of test bacterial strain CD105LC2 (B), 106 PFU/larva of the 4-phage cocktail (P), a co-infection of the 4-phage cocktail and bacteria (P+B) or remained untreated (-) at the time points shown. Insects were culled at each relevant time point by incubating them at −20 °C for 20 min.