Figure 5.

E2 modified EMT marker distribution and immunoreactivity in human GBM-derived cells. ZO-1, N-cadherin, and vimentin immunostaining in (A) U251 and (B) U87 cells treated with 17β-estradiol (E2, 10 nM) and vehicle (V, 0.01% cyclodextrin) for 48 h. Representative images were captured under a fluorescence microscope at a magnification of 400×. (C) EMT marker expression measured as a fluorescence density. Results are expressed as the mean ± standard error of the mean (SEM); n = 3; * p < 0.05 vs. V.