Figure 6.

E2 increased migration and invasion of human GBM-derived cells. (A,B) Wound healing assays were performed in U251 and U87 cells treated with 17β-estradiol (E2, 10 nM) and vehicle (V, cyclodextrin 0.01%). Representative images of wound closure at 0, 10, and 20 h and quantification of the wound area are shown. (C,D) Transwell assays were carried out in both cell lines. Quantification of cells staining with 0.1% crystal violet dye shows the number of invasive cells. Results are expressed as the mean ± standard error of the mean (SEM); n = 3; * p < 0.05 vs. V.