Table 5.

Degrees of statistical significance of Chi2 tests comparing the percentage of positive C. parvum real-time PCR according to the pretreatment/extraction protocols for the concentrations of 10, 50, and 100 oocysts/mL.

	EM			IN			QF			QP			ZR			MPG			MP
	10	50	100	10	50	100	10	50	100	10	50	100	10	50	100	10	50	100	10	50	100
EM	na	na	na	ns	*	ns	***	***	***	ns	ns	ns	ns	ns	ns	ns	ns	ns	ns	**	ns
IN	ns	***	ns	na	na	na			**	*	ns	ns	**	*	ns	ns	ns	ns	ns	ns	ns
QF	***	ns	***	**	*	**	na	na	na			***	***	***	***	***	***	***	**	ns	**
QP	ns	ns	ns	*	ns	ns	***	**	***	na	na	na	ns	ns	ns	ns	ns	ns	*	ns	ns
ZR	ns	ns	ns	**	*	ns	***	***	***	ns	ns	ns	na	na	na	ns	ns	ns	**	***	ns
MPG	ns	ns	ns	ns	ns	ns	***	***	***	ns	ns	ns	ns	ns	ns	na	na	na	ns	ns	ns
MP	ns	**	ns	ns	ns	ns	**	ns	**	*	ns	ns	**	**	ns	ns	ns	ns	na	na	na

* (p ≤ 0.05), ** (p ≤ 0.01), *** (p ≤ 0.001), ns, not significant, na, not applicable. EM, Nuclisens^® EasyMAG^®, bioMérieux; IN, ELITE InGenius^®, ELITechGroup; QF, QIAamp Fast DNA Stool Mini Kit^®, Qiagen; QP, QIAamp Power Fecal DNA Kit^®, Qiagen; ZR, Quick DNA Fecal/Soil Microbe Miniprep^®, ZymoResearch; MPG, MagnaPure 96^® with grinding, Roche Diagnostics; MP, MagnaPure 96^® without grinding, Roche Diagnostics.